αS PFF Uptake Protocol
Scott Vermilyea
Published: 2024-06-26 DOI: 10.17504/protocols.io.e6nvw1e79lmk/v1
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Abstract
This protocol details the uptake of αS PFF treatement.
Steps
αS PFF Uptake Protocol
1.
Exchange fresh culture media to cells.
2.
Prepare αS PFF working solution.
- Frozen stock is
5, dilute to0.25in sterile PBS followed by sonication (1 Sec ON/ 1 Sec OFF) for0h 2m 0swith 20% amplitude.
3.
Add αS PFF to cells for a final concentration of 4.
4.
Incubate for desired duration prior to lysate collection.
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